Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Enzyme Aggregates

The immobilization of horseradish peroxidase in the form cross-linked enzyme aggregates (HRP–CLEAs) was optimized by three parameters full factorial experimental design at two levels and degradation an azo dye tested. optimal conditions were estimated as 0.06 mg enzyme/mL (0.96 U), 3 % (v/v, Glutaraldehyde)) cross-linker ratio, 6 /mL Bovine Serume Albumine amount, respectively. effects variables analysed responce surface plots concentration effect insignificant for tested levels. maximum CLEAs activity 0.2188 U kinetic (Km Vmax) 0.0314 mM 0.1044 mM/min while calculated values soluble 0.468 mM/min, Hydrogen peroxide has inhibitory on aggregate activities higher amounts than 0.012 mM. Under conditions, a textile dye, Reactive Blue 160 completely oxidized within 90 h temperatures also showed promising results degradation.